![]() ![]() To measure the height of an integration, you start at the bottom of the integration curve where it's flat, and measure to where the curve goes flat again. Integration curves and hydrogen peaks on an 1H NMR spectrum. Keeping this in consideration, how does NMR measure integration? It is used to determine the ratio of hydrogens that correspond to the signal. It corresponds to the amount of energy absorbed or released by all nuclei participating in chemical shift during the nuclear spin flip process. Of course, you can also make your 2D spectrum with a single color (no ramp).Integration is the measurement of peak areas on the NMR spectrum. You will find below two overlaid spectra with different colours (one red and the other purple): It is also possible to change the colours of the 2D overlaid spectra by following the above procedure (but changing the colours). ![]() You can see below an expanded signal after having applied the resulting palette (obtained in the previous step): Please take a look at the screenshot below for further information. Do the same for the negative peaks in this case the contour number 11 will be 'light blue' and the number 20 will be 'dark blue'. Finally, select the colour of the contour number 10 as 'light green' and check the mark. For example, imagine that you have 10 positive contours, then you will need to select the colour of the contour 1 as 'dark green' (for example) and then check the corresponding mark at the right side. In this case, you will only need to select in the '2D Spectrum Palette' dialog box the first and the last colour of the positive peaks and the same for the negatives. ![]() Let´s take a look at another situation: How to make all positive contours a scale of greens and all negative contours a scale of blues? If you have 20 levels, do the same with the 20th level and so one If you have 10 levels, check the 10th level of the palette and then click on the light-grey rectangle (just at the left side of the check mark) and select the black colour. Select 'Grayscale' on the 'Template' colours scroll down menu 2. If you want to obtain monochrome printouts, in pure black and white with no grayscale: 1. Next click on the 'Palette Editor' button to display the '2D-Spectrum Palette' dialog box, where you will be able to customize the desired palette (Name, Level Growth, Colors, Number of Levels, etc.): ![]() Just open a 2D spectrum, double click on the spectral window to display the 'Properties' dialog box and then select for example 'Custom 2' in the 'Palette scroll down menu. No worries, it is possible to customize the desired 2D spectrum palette. ![]()
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